Cytotoxic Activity of Mixture from Cymbopogon nardus, Curcuma aeruginosa and Curcuma zanthorrhiza^s Essential Oil Against MCF-7 Cells
1 Rizki Fitria, 2 Djarot Sasongko Hami Seno, 3 Bambang Pontjo Priosoeryanto, 2.4 Waras Nurcholis

1 Department of Chemistry, Lambung Mangkurat University, Indonesia
2 Department of Biochemistry, IPB University, Indonesia
3 Department of Veterinary Clinic Reproduction and Pathology, IPB University
4 Tropical Biopharmaca Research Center, IPB University, Indonesia

Abstract

Plants produce a variety of secondary metabolites, one of them in the form of essential oils that contain a variety of volatile compounds and are useful for humans. Cymbopogon nardus, Curcuma aeruginosa Roxb and Curcuma zanthorrhiza are contain volatile compounds from essential oils that can inhibit the proliferation of cancer cells. This research aimed to explore the antiproliferative activity of a mixture of C. nardus, C. aeruginosa Roxb and C. zanthorrhiza^s essential oils with different ratios against breast cancer Michigan Cancer Foundation (MCF-7) cells in vitro. There were four formulations made: Formulation 1 (C. nardus and C. aeruginosa), formulation 2 (C. nardus, C. aeruginosa and C. zanthorrhiza), formulation 3 (C. aeruginosa and C. zanthorrhiza) and formulation 4 (C. nardus and C. zanthorrhiza). Antiproliferation test was carried out with total cells method using trypan blue and cells were calculated using a microscope. The results showed that the Inhibitory Concentration 50 (IC50) value of each formulation was 162.3 ppm- 160.1 ppm- 168.3 ppm and 148.7 ppm, respectively. Inhibition percent of these formulas against Vero normal cells were 10%- 14%- 6.8% and 4.3%, respectively. The best formulation that can be potentially developed as an inhibitor for cancer cell proliferation was formulation 4.

Keywords: antiproliferation, breast cancer, essential oils, formulation, MCF-7

Topic: Biochemistry