ISOLATION AND SCREENING OF POTENTIAL ACTINOMISET FROM MANGROVE SOIL WONOREJO SURABAYA
Endah Prayekti, Viera Nu^riza Pratiwi, Nathalya Dwi Kartika Sari

Universitas Nahdlatul Ulama Surabaya

Abstract

Many studies support the ability of actinomycetes to produce antibiotics, but the emergence of antibiotic-resistant bacteria requires new sources of antibiotics. The potential for actinomycete exploration is very promising when carried out on mangrove soils. This is due to the unique function of the mangrove ecosystem, namely as a barrier to the spread of toxins from land to water. The purpose of this study was to isolate and screen actinomycetes from the mangrove soil. Soil samples were taken from mangrove soil at a depth of 10 cm from the surface. The isolation medium used was Actinomycete Isolation Agar (AIA) media supplemented with the antibiotic amoxicillin 2mg/100mL. Pure cultures of actinomycetes were tested for their inhibitory ability against Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli. The results of the isolation process obtained three actinomycetes that have the potential to inhibit the test bacteria. The three actinomycetes isolates showed a strong inhibitory ability in the range of 1.5cm against Staphylococcus aureus. The conclusion of this study, the three types of actinomycetes have the ability to inhibit the test bacteria, with the greatest potential shown by isolate C1.

Keywords: Actinomycetes, Screening, antibacterial, mangrove soil, Wonorejo Surabaya

Topic: Microbiology