Allium sativum Methanolic Extract Effect on Urease Production Using New Isolated Alkaline Bacteria
Fellycita Kartika Afriliona, Danu Risqi Yudiargo, Fida Alya Putri Rimansa, Frida Ayu Wahyuwati, Izzatul Islami, Karimatul Millah, Nadhifah Astari Putri, Endry Nugroho Prasetyo*

Gedung H, Department of Biology, Faculty of Science and Data Analytics, Institut Teknologi Sepuluh Nopember, Kampus ITS, Keputih, Sukolilo, Surabaya 60111, Indonesia
*endry[at]bio.its.ac.id

Abstract

Urease is an enzyme produced by ureolytic microorganisms that can hydrolyzes urea to ammonia and carbon dioxide. Microbial urease has also been studied in clinical samples because it is associated with the level of pathogenic microorganisms virulences. Urease producing microbes can be isolated in different ecosystems such as soils and geological formations. This study aims to determine the optimum activity of urease production using gram negative bacteria in the presence of natural inhibitor (Allium sativum extract). The production of the enzyme was determine using Nessler method. The result showed that low concentration of Allium sativum inhibited the production of urease activity at 5000 ppm concentration.

Keywords: Allium sativum- Inhibitor- Urea- Urease- Ureolytic

Topic: Biotechnology