DNA Isolation and Amplification of the Cytochrome b Gene from Drosophila melanogaster
Ahmad Fauzi1, a), Diani Fatmawati1, b), Diana Khoiroh1, c), Siti Zubaidah2, d), Hendra Susanto2, e), and Poncojari Wahyono1, f)

1 Department of Biology Education, Faculty of Teacher Training and Education, Universitas Muhammadiyah Malang (Jl. Raya Tlogomas 246 Malang 65144)
2 Department of Biology, Faculty of Mathematics and Natural Science, Universitas Negeri Malang (Jl. Semarang No. 5, Malang 65145, Indonesia).

Abstract

Drosophila melanogaster is the most popular model organism in the field of Biology. Unfortunately, in Indonesia, the utilization of this organism is still far from optimal. This study aimed to isolate DNA and amplify the cytochrome b gene of D. melanogaster. The stages of the research were 1) culturing D. melanogaster- 2) sample treatment process- 3) DNA extraction and purification- 4) test the results of DNA extraction- 5) cytochrome b gene amplification- and 6) analysis of cytochrome b gene amplification results. G-Spin TM Total DNA Extraction kit successfully isolated the genome of D. melanogaster. In a thermo-cycler that was set with a lid temperature of 104C, pre denaturation 94C for 5 minutes, denaturation 94C for 1 minute, annealing for 1 minute, elongation 72C for 2 minutes, and post elongation 72C for 5 minutes, the cytochrome b gene was amplified at annealing temperatures of 57C and 58C. These findings are expected to initiate and encourage wider use of D. melanogaster in various research fields in Indonesia, particularly in molecular studies.

Keywords: cyt b gene, DNA extraction, fruit files, model organism, PCR amplification

Topic: Zoology