Effect Black Soybean Tempeh Extract against Liver Enzymes in Streptozotocin-Induced Diabetic Rats
Esha Ardiansyah1, Clara Kartika Aprilia1, Khoirum Imawati1 Mochammad Fitri Atho^illah2, Siti Nur Arifah1, Agung Witjoro1, Sri Rahayu Lestari1, Abdul Gofur1*)

1 Department of Biology, Faculty of Mathematics and Natural Sciences, State University of Malang, Jalan Semarang no. 5, Malang 65145, East Java, Indonesia.
2 Department of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University, Malang 65145, East Java, Indonesia.

*Corresponding author: abdul.gofur.fmipa[at]um.ac.id

Abstract

Abstract. Type 2 diabetes is caused by insulin resistance, which is characterized by hyperglycemia and obesity. Obesity is caused by high-fat diet or high-sucrose, which causes fatty liver, so that lipid metabolism increases as well as liver mitochondrial dysfunction and insulin resistance. Liver mitochondrial dysfunction releases liver enzymes into the bloodstream and blood levels of SGPT and SGOT increase. This study aimed to determine the black soybean tempeh exract on lipid profiles and liver enzymes. The rats were made type 2 DM by feeding high-fat diets, 10% sucrose and then were injected intraperitoneally with multiple low doses of streptozotocin. The rats were treated for 30 days with 4 treatment groups: normal, negative control, positive control and treatment. At the end of treatment, rats were sacrificed and blood was taken from the heart and liver was collected. Blood is centrifuged to get blood serum. Serum levels of profile lipid and liver enzymes were measured by spectrophotometric methods. One-way ANOVA analysis showed that BST had a significant effect (p<0.05) on increasing HDL by 29 mg/dL and decreasing LDL by 11.5 mg/dL, total cholesterol by 75.5 mg/dL, triglyceride by 104 mg/dL, glucose by 98 mg/dL, SGPT by 40 U/L and SGOT levels by 178 U/L.

Keywords: Keywords: black soybean tempe extract, HDL, LDL, total cholesterol, triglyceride, glucose, SGPT, SGOT, DM Type 2.

Topic: Zoology