Enzymatic and Microbial Biogrouting Using Various Substrates
Fida Alya Putri Rimansa, Danu Risqi Yudiargo, Fellycita Kartika Afriliona, Frida Ayu Wahyuwati, Izzatul Islami, Karimatul Millah, Nadhifah Astari Putri, Endry Nugroho Prasetyo*

Gedung H, Department of Biology, Faculty of Science and Data Analytics, Institut Teknologi Sepuluh Nopember, Kampus ITS, Keputih, Sukolilo, Surabaya 60111, Indonesia
*endry[at]bio.its.ac.id

Abstract

Urease is an hydrolising enzyme produce by ureolytic microorganism which convert urea into ammonia and carbon dioxide. It is synthesized by a wide range organisms like bacteria, fungi, and some plants. Source of urease-producing microbes is generally distributed in soil and aquatic environments. Urease applications is used in medicine, agriculture and geotechnics. Nowadays, urease is as well extensively used as new alternative replacement of grouting material. The aim of the study was to determine effect of various substrate for grouting applications using enzyme and whole microbes. The result showed that highest strength of grouting materials was achieved using enzyme applications comparing to whole microbes.

Keywords: Urease- Biogrouting- Alternative- Substrate- Whole microbes

Topic: Biotechnology