Preparation of Microbore Polymer-based Monolithic Columns as Chiral Stationary Phases for Liquid Chromatography Akhmad Sabarudin, Suci Amalia, Stevin C Angga, Bhisma W Khabibi
Department of Chemistry, Faculty of Science, Brawijaya University, Jl Veteran 65145, Malang, Indonesia.
Abstract
Enantiomer molecules have different structure conformations which exhibit different beneficial biological activities as well as pharmacological effects. Separation of enantiomers requires great effort since ordinary chromatographic separation could not distinguish these molecules. In this work, poly-(glycidyl methacrylate-co-ethylene glycol dimethacrylate) (poly-(GMA-co-EDMA)) and poly-(glycidyl methacrylate-co-trimethylolpropane trimethacrylate) (poly-(GMA-co-TRIM)) columns were prepared by in-situ copolymerization inside a PEEK tubing using porogens of 1-propanol, 1,4-butanediol and water at appropriate ratio in the presence of AIBN as a radical initiator (1% of the total monomer). These monoliths were modified with ethylenediamine-beta-cyclodextrin (EDA-B-CD) for separation of chiral compounds. Additionally, metal-mediated monolithic molecularly imprinted polymers (MIPs) columns were prepared using the ionic liquid of [BMIM]BF4 as the main pore-forming agent, and transition metal ions as the pivots, whereas an (R)-(+)-citronellal or an (R)-(+)-pulegone was used as a template, and 4-vinylpyridine and EDMA or TRIM as a cross-linker.
Keywords: Chiral Separation, Polymer, Monolith, Chromatography, Metal pivot
