ISOLATION AND CHARACTERIZATION OF MICROBES FROM TANNING WASTE SEDIMENTS THAT HAVE THE POTENTIAL TO BE BIOREMEDIATION AGENTS OF CHROME WASTE Mohamad Febby Setyawan1, a) Eli Hendrik Sanjaya1, b)
Departemen of Chemistry, Faculty Of Matematic and Natural Science, Universitas Negeri Malang
Abstract
Abstract. The tanning industry is one of the sources of pollutants in Indonesia. Various kinds of contaminants produced by the Tanning Industry are hazardous substances, including heavy metals. Chromium is one of the most dangerous heavy metals, its hexavalent states are even more toxic than its trivalent states. Bioremediation is one way to reduce heavy metal contamination, including chromium hexavalent. One solution to overcome Cr(VI) pollution is using Cr-resistant bacteria. This study aims to explore Cr-resistant bacteria from tanning sediment waste. Screening of Cr(VI) resistant microbes was performed using LB Agar media containing Chromium(VI) 50 ppm. Two isolates are coccus-shaped Gram-negative bacteria (Isolates C and B) and Gram-positive bacteria in the form of coccus (Isolate A). Isolate A can withstand Cr(VI) concentrations of up to 400 ppm and has a reduced ability of Cr(VI) up to 74% at concentrations of 200 ppm. Meanwhile, C and B isolates can survive at Cr(VI) concentrations up to 300 ppm and can absorb Cr(VI) up to 68% at a concentration of 100 ppm.
