Biodiversity of Anammox Microbial in the Malang Karangkates Resevoir and Its Activities as A Degradation Agent for Ammonia Rich Waste. Abdul Khafid Syahroni1,a), Eli Hendrik Sanjaya1,b), Much. Sayfulloh Alwy1,c), Mohd Fadhil MD Din2,d), Hong Chen3,e), Sitoresmi Prabaningtyas4,f), Suharti1,g), Mieke Alvionita1,h), and Anugrah Ricky Wijaya1,i).
1) Chemistry Departemnt, Faculty of Methematics and Natural Sciences, Universitas Negeri Malang Indonesia.
2) Civil and Environmental Enginering, University Teknology Malaysia. Johor Baru, Malaysia
3) School of Hydrolic Engineering, Changsha University of Science and Technology, China
4) Biology Departement, Faculty of Mathematics and Sciences, Universitas Negeri Malang, Indonesia
Abstract
Nitrogen is an important element that plays a role in the cycles in the world. The nitrogen cycle is the most important cycle for living things after the carbon cycle. One of the newest processes in the nitrogen cycle is the anaerobic ammonia oxidation (anammox) process. Anammox is a process when nitrite is used as an electron acceptor in the conversion of ammonium to nitrogen gas with the help of anammox microbes. This study aims to isolate anammox bacteria from sediments from Karangkates resevoir using the Bottle Batch Reaktor method, as well as testing the activity of anammox bacteria in reducing excess ammonia in synthetic wastewater containing amonia using colorimetry method. This study also identifying the biodiversities of anammox microbes in sediment sample using Next Generation Squencing (NGS) testing. The stages in this research are: (1) Preparation of sediment samples and water samples from Karangkates resevoir, (2) water sample parameters analysis, (3) identification of microbial biodiversity involved in nitrogen cycle using Next Generation Squencing (NGS), (4) cultivation of samples in Bottle Batch Reactor and (5) analyse the performance microbes in removing ammonia. The NGS analysis obtained Anammox microbes from the Kuenen species, namely Candidatus Kuenenia Stuttgartiensis with an abundance of 0.07%. Apart from the plantomycetes phylum, it also found several other microbes which usually involved in the nitrogen cycle, such as Proteobacteria 40%, Bacteroidetes 4%, Planctomycetes 6%, Chloroflexi 1%, Nitrosomonas 0.03%, Nitrosospira 0.1%, Nitrospiraceae 0.5% and Pseudomonas 0.2%. The average of amonia removal in this study was 18 % mL/Day.
