Filogenetic Analysis of Indigenous Wood Rot Fungus From East Java and Their Potential as Ligninase Producers in Kirk Medium Evi Susanti1,2*, Suharti1,2, Eli Hendrik Sanjaya1,2, M. Irhamul Iqbal1 dan Avin Arinta1
1Chemical Department, Faculty of Mathematic and Natural Science, Universitas Negeri, Malang, East Java, Indonesia
2Biotechnology Department, Faculty of Mathematic and Natural Science, Universitas Negeri, Malang, East Java, Indonesia
Abstract
Exploration of indigenous wood rot fungus (WRF) producing ligninase from tropical areas such as Indonesia has not been widely carried out. Whereas Indonesia is known to have microbial mega biodiversity, so it is possible that there could be found the novel WRF that capable of producing distinctive ligninase. KLUM1, KLUM2 are indigenous KPK isolates obtained from the Kediri Cocoa Plantation and PnUM from the forst of Pujon Malang at East Java Indonesia. Kinship analysis based on microscopic observations is difficult to carry out at the species level. So in this study, a phylogenetic analysis was carried out to the species level based on the Internal Transcribed Sequence (ITS) using ITS4-ITS5 primers and identification of biochemical potentials including identification of lignin peroxidase (LiP), manganese peroxidase (MnP) and laccase produced in standard Kirk medium. The isolates KLUM1 and KLUM2 were suspected to be novel WRF strains because KLUM1 only had 99% similarity with Myceliophtora thermophilia and KLUM2 of 97% with Thermothelomyces guttulata, while PnUM was identified as Phialemonium inflatum because it had 100% similarity. All three have the ability to produce LiP and MnP which are more dominant than laccase in standard Kirk medium. Thus, the three isolates have the potential to be explored as a source of ligninase enzymes. The exploration of the ligninase ability of these three isolates in various mediums will be interesting for further research in an effort to produce ligninase that is unique and has high activity.
