Antioxidant Activity Of Ethanol Extract Ulva sp And Gracilaria sp From Sayang Heulang Using FRAP (Ferric Reducing Antioxidant Power) Method Sri Maryam,1 Nurul Novianti,1 Lita Mulyani,1 and Windy Widowaty 2,*)
1) Departement of Pharmacy, Faculty of Mathematics and Natural Science, Al-Ghifari University Jl. Cisaranten Kulon No. 140 Bandung
2) Departement of Food Technology, Faculty of Agroindustrial Technology, Al-Ghifari University Jl. Cisaranten Kulon No. 140 Bandung
Ulva sp and Gracilaria sp are found on the coast of Sayang Heulang beach and have the potential as natural antioxidants. This study aims to determine the antioxidant capacity of the two types of seaweed using the FRAP (Ferric Reducing Antioxidant Power) method. Ulva sp and Gracilaria sp were taken from Sayang Heulang Beach in March 2022. Each type of seaweed was extracted using 70% ethanol for 3x24 hours. The extract was then concentrated using a rotary evaporator for further analysis. The results of phytochemical screening showed that the ethanolic extract of Ulva sp was positive for flavonoids, tannins, saponins, and alkaloids, while the Gracilaria sp extract was positive for alkaloids, flavonoid tannins, steroids, and saponins. The antioxidant capacity of Ulva sp and Gracilaria sp ethanol extracts was carried out using the FRAP method with ascorbic acid used as standard. Each seaweed extract was measured using a UV-Vis spectrophotometer with a wavelength of 694 nm. The measurement results were expressed as mg equivalent of ascorbic acid/gram extract (mg AAE/gr sample). The analysis showed that the ethanolic extracts of Ulva sp and Gracilaria sp had antioxidant capacity values of 1.8 mg AAE/gr sample and 5.50 mg AAE/gr sample, respectively. This study is expected to provide information about the potential bioactivity of seaweed originating from the Sayang Heulang beach, Garut, especially Ulva sp and Gracilaria sp.
