HISTOPATHOLOGICAL ASSAY WHITE RAT GASTER (Rattus norvegicus) EXPOSED TO FORMALIN AND TREATED GRAPE SEED ETHANOL EXTRACT (Vitis vinifera)
Arifiani Agustin Amalia,1, b) Ana Hidayati Mukaromah,1, a) Mudyawati Kamaruddin,1, c)

1) Masters Program in Clinical Laboratory Science, University of Muhammadiyah Semarang. Street of Kedungmundu Raya No.18 Semarang, Central Java, Indonesia.
a) Corresponding author: ana_hidayati[at]unimus.ac.id
b) Electronic mail: arifiani.agustinamalia[at]gmail.com
c) Electronic mail: mudyak706[at]gmail.com

Abstract

The use of formalin as a food preservative can cause a decrease in the height of the gastric mucosa. Formalin works by inhibiting gastrin secretion which results in an increase in HCl secretion in the stomach. The increase in HCl secretion in the stomach causes an increase in ROS levels which can cause oxidative stress which causes lipid peroxidation in the cell membrane, resulting in gastric histopathological changes. Grape seed extract contains flavonoid compounds as antioxidants. The purpose of this study was to determine the effect of grape seed extract therapy on the improvement of gastric histopathology in white rats. White rats were probed using formalin at a dose of 25mg/kg orally once a day for 21 days. This study used 5 treatment groups, namely a negative control group, a positive control group (formalin exposure), and a grape seed extract therapy group at a dose of 0.035- 0.070, and 0.140g/mL/rat/day given orally once a day for 21 days. The gastric histopathology test with HE staining was analyzed using the Barthel Manja scoring method and analyzed by the Kruskal Wallis statistical test to determine the differences between each group. The results obtained were gastric mucosal epithelial integrity scores for a dose of 0.035- 0.070 and 0.140g/mL, respectively, namely 2.4- 2.0- and 0.6 which showed that grape seed extract therapy significantly (p<0.01) could improve histopathology in gastric mucosal epithelial cells. The conclusion of this study shows that grape seed extract therapy can improve the gastric histopathological picture of white rats, so that grape seed extract can be used as an alternative therapy in diseases caused by formalin exposure.
Keywords: Gaster, Histopathology, Malondialdehyde, Formalin.

Keywords: Formalin, Gaster, Histopathology, Malondialdehyde.

Topic: Biomaterials, Tissue engineering, Pharmaceuticals Industry