Toxicity Analysis of Flavonoid Compounds in Majapahit Leaf Extract (Crescentia cujete) Against Various Cancer Cell Lines Using CLC-pred Tools Program
Fatimah Fatimah 1,a), Rahma Diyan Martha2,b), and Danar Danar 3,c)

1Department of Medical Laboratory Technology, STIKES Karya Putra Bangsa. Tulungagung, Indonesia.
2Department of Pharmacy, STIKES Karya Putra Bangsa, Tulungagung, Indonesia.
3Department of Chemistry, Faculty of Mathematics and Science, Universitas Negeri Malang, Indonesia.

Abstract

The Majapahit plant (Crescentia cujete) is one of the plants that originated from Central America, now widely encountered in Indonesia. Utilization of the Majapahit plant (Crescentia cujete) in Indonesia is still very limited, even though in its home country this plant was used as one of the ingredients of traditional medicine. One of the plant organs that are often used is the leaf. Based on this fact, we conducted a study to determine the potential of Majapahit leaves as an anticancer agent. In this study, we analyzed flavonoid compounds in Majapahit leaf extract (Crescentia cujete) using LCMS followed by in silico analysis of flavonoid compounds with various cancer cell lines using the CLC-pred tools program.
Based on compound analysis using LCMS, there are 10 types of compounds in Majapahit leaf extract (Crescentia cujete) that are included in the flavonoid group, namely- Quercetin, Acacetin 7- rutinoside, Kaemferol 3-O rhamnoside, Kaemferol 3- [6^-(3- hydroxy- 3- methylglutaryl)glucoside], Kaemferol 3-[6^-(3- hydroxy-3- methylglutaryl)glucoside] -7-glucoside, Didymin, Diosmin, Hesperidin, Narirutin 4^- glucoside, Rutin. The flavonoid compound that had the largest percentage was Kaemferol 3-O rhamnoside at 3.90 percent. The identified flavonoid compounds were analyzed in silico using the CLC-pred tools program to determine the ability of the compounds to be toxic to cancer cell cultures. Based on in silico analysis using CLC-pred tools, it is known that each of the identified anticancer potential compounds has different cytotoxic abilities against different types of cell lines.

Keywords: Majapahit leaves, LCMS, anticancer, insilico

Topic: Organic Chemistry