Analysis constituents of ethyl acetate fraction of Curcuma xanthorrhiza and Curcuma zedoaria (Berg.) Roscoe) and the potency as anticancer
Siti Mariyah Ulfa*, Andrian Sucahyo, Nur Fitriana, Widodo, Muhaimin Rifai

Chemistry Department, Faculty of Mathematics and Natural Science, Brawijaya University

Abstract

A long history of the uses of herbal medicine in Asia, including Indonesia comes from the folklore with limited information about the constituents responsible for the pharmacological effects. Here, we reported the extraction of Curcuma xanthorrhiza (CX) and Curcuma zedoaria (Berg.) Roscoe) (CZ) root using alcohol followed by solvent partition using ethyl acetate and determined the active constituent for anticancer. The partition of CX and CZ crude extract gave 67.93% and 64.3% ethyl acetate fraction, respectively. Toxicity screening of CX and CZ ethyl acetate fraction showed that both curcumas had the high toxicity. Purification using SiO2 column gave 9 subfractions of CX and 11 subfractions of CZ. Bioactivity analysis using T47D breast cancer cell line showed a prospective result. Subfraction CX-F3 and CX-F4 have the highest toxicity with IC50 54.8 +/- 1.5 ug/mL and 59.3 +/- 3.0 ug/mL, respectively. Furthermore, subfraction CZ-F1, CZ-F2, CZ-F4, CZ-F5, and CZ-F8 have IC50 between 50-60 ug/mL. Based on this result, the constituent in the ethyl acetate fraction of CX and CZ suggested to have a high potency as anticancer agent.

Keywords: anticancer, ethyl acetate fraction, Curcuma zeodaria, Curcuma xanthorrhiza, T47D cell line

Topic: Organic Chemistry