Optimization of Culture Medium of Production Cellulolytic Enzyme Isolated from Termite Gut in Indralaya Peatlands, Indonesia
Dwita Oktiarni (1, 2*), Hermansyah (3**), Eddy Ibrahim (4), Marsi (5), Hasanudin(3), Miksusanti(3), Dede Heri Yuli Yanto(6), Nanik Rahmani(7), Getari Kasmiarti(8)

1) Doctoral Program of Mathematics and Natural Sciences, Graduate School, Universitas Sriwijaya. Jl. Palembang-Prabumulih Km. 32, Indralaya, Ogan Ilir 30662, South Sumatra, Indonesia. Tel.: +62-711-580269 ext. 580056, Fax.: +62-711-580056.
*dwita.oktiarni[at]unib.ac.id
2) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Bengkulu, Jl. WR. Supratman Kandang Limun, Muara Bangka Hulu, Bengkulu 38371, Bengkulu, Indonesia.
3) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Sriwijaya. Jl. Palembang-Prabumulih Km. 32, Indralaya, Ogan Ilir 30662, South Sumatra, Indonesia. Tel.: +62-711-580269 ext. 580056, Fax.: +62-711-580056
**hermansyah[at]unsri.ac.id
4) Department of Mine, Faculty of Engineering, Universitas Sriwijaya. Jl. Raya Palembang-Prabumulih Km. 32, Indralaya, Ogan Ilir 30662, South Sumatra, Indonesia
5) Department of Soil, Faculty of Agriculture, Universitas Sriwijaya. Jl. Raya Palembang-Prabumulih Km. 32, Indralaya, Ogan Ilir 30662, South Sumatra, Indonesia
6) Research Center for Biomaterials, Indonesian Institute of Sciences, Cibinong Science Center, Jl. Raya Bogor Km. 46 Cibinong 16911, West Java, Indonesia
7) Research Center for Biotechnology, Indonesian Institute of Sciences, Cibinong Science Center. Jl. Raya Bogor Km. 46, Cibinong 16911, West Java, Indonesia
8) Doctoral Program of Environmental Science, Graduate Program Universitas Sriwijaya, Jalan Padang Selasa No.524, Bukit Besar, Palembang 30139, South Sumatra, Indonesia.

Abstract

Bioethanol is an energy source that has the potential to be developed as an eco environmentally energy. Enzymatic process can be used for cellulosic biomass to to produce bioethanol. Termite is the organisms that consume cellulose as a food source. In this study, cellulolytic bacteria from termite gut were optimized to increase the cellulolytic enzyme production. Several factors can affect the growth and production of cellulase enzymes. In this research, optimizing the culture medium followed by different carbon sources, various concentration of carbon sources, diverse nitrogen sources, various concentration of nitrogen sources, pH, temperature, and agitation were examined. The result showed that medium optimal with higher enzyme activity was obtained in the addition of a carbon source of fructose with concentration of 3.5%, nitrogen source of sodium nitrate with concentration of 2%, pH of 5, temperature of 45oC, and agitation of 150 rpm.

Keywords: bioethanol, cellulolytic enzyme, termite

Topic: Biochemistry