Phytochemical and Toxicity Tests On Aedes Aegypti Larva and Antioxidant Activities of Methanol Extracts of Roots, Fruit and Leaves of Bintaro (Cerberra Manghas)
Khaerul Rahman, Usman

Program Studi Kimia, FKIP, Universitas Mulawarman

Abstract

Bintaro plant is a tropical plant that has many benefits, one of the benefits is that it is used as a pest repellent. This plant has toxic compounds that have the potential as antioxidants, anti-inflammatory, anti-bacterial, anti-fungal and so on. This study aims to determine the secondary metabolite compounds by phytochemical tests, to determine the activity of toxicity against Aedes aegypti and to determine the antioxidant activity of methanol extract of the root tissue, fruit and leaves of the Bintaro plant. Phytochemical test analysis was carried out by means of qualitative tests, such as alkaloids, flavonoids, phenolics, steroids, triterpenoids, saponins, and tannins tests. As for the antioxidant activity test using the DPPH method, absorption was measured at a wavelength of 517 nm. The results of the phytochemical test showed that the methanol extract of bintaro roots and fruit contained alkaloids, phenolic compounds, and triterpenoids, while the methanol extract of bintaro leaves contained alkaloids and triterpenoids. The results of the toxicity test against the Aedes aegypti mosquito methanol extract of the root tissue with an LC50 of 33,464 4,497 ppm, fruit tissue with an LC50 value of 16,157 0,296 ppm and leaf tissue of the Bintaro plant with an LC50 value of 24,496 1,851 ppm. Based on the LC50 value, it can be concluded that the methanol extract of the root tissue has a moderate level of toxicity while the fruit and leaf tissue has a high level of toxicity. Then the results of the antioxidant activity test of the methanol extract of the bintaro root had an IC50 value of 211.375 5 ppm, the antioxidant activity of the methanol extract of the bintaro fruit had an IC50 value of 87,013 0,022 ppm, while the bintaro leaf had an IC value of 254,0313 5,96 ppm. Based on the IC50 value, it can be concluded that the methanol extract of bintaro roots and leaves has antioxidant activity in the weak category, while the methanol extract of bintaro fruit has a strong

Keywords: Phytochemical test, toxicity, antioxidant, secondary metabolite, Bintaro plant

Topic: Organic Chemistry