FINE GROWTH ON EXPLANTS OF MANGGIS LEAF (Garcinia mangostana L.) PLANTED BY IN VITRO
Siti Hafizah Al Khairiyah Bintang (a*), Fauziyah Harahap (b), Melva Silitonga

a) Postgraduate Student, State University of Medan
Jalan Willem Iskandar/Psr. V 10 Medan Estate, Indonesia
*khairiyah.bintang96[at]gmail.com
b) Lecture of Biology Education Program, State University of Medan
Jalan Willem Iskandar/Psr. V 10 Medan Estate, Indonesia

Abstract

This study was conducted to determine the effect of growth regulators (ZPT) on callus induction of mangosteen (Garcinia mangostanal. ) from leaf sources grown in vitro. The study was carried out from February 2022 to June 2022. This study used a completely randomized design (CRD) with 2 treatment factors, namely: (a) the first factor concentration of 2,4D consisted of 4 levels, namely: 0 ppm, 1, 5 ppm, 3 ppm and 4.5 ppm. (b) the second factor of vitamin concentration consists of 3 levels, namely: 0 ppm, 1 ppm and 2 ppm. Callus appears first begins with the warping of the explant. Explants that grow are characterized by the appearance of white spots on one or several parts of the explants. The data from the observation of the fastest callus formation in the MediaV2+D3 treatment was the 2nd week. Meanwhile, the time for callus to appear was at the latest in the V2+D1,5 media treatment, which was the 12th week. The height of the callus pile observed at week 12 after planting was carried out by calculating the callus height that grew from all explants. The results of data analysis showed that the V1+D0 media treatment resulted in the highest number of Callus Pile Height 0.60 at 12 WAP observations. The lowest callus heap height yield was obtained from treatment with an average number of callus 0.33 roots. The average growth of the Callus Pile Height responded to an increase in the number of roots. The results of data analysis showed that the V2+D0 media treatment resulted in the highest number of callus widths of 1.36 at 12 WAP observations. The results of the lowest number of callus widths were obtained from the treatment of Media V0+D1,5 and V2+D1,5 with an average number of callus widths of 0.06. The average growth of callus width gave a response to an increase in the number of leaves. Callus color is influenced by growth regulators.

Keywords: In vitro, ZPT, Vitamin, 2.4 D

Topic: Mathematics,Science and Nursing Education